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Dinophysis spp. cells concentrated from nature for experimental purposes, using size fractionation and reverse migration ArchiMer
Maestrini, Serge Y; Berland, Brigitte R; Grzebyk, Daniel; Spano, Anna-maria.
A method based on size fractionation plus reverse phototactic migration was used to collect large Dinophysis spp.-dominated populations. Cells were active and survived handling and transportation. Experiments involving various treatments for establishing permanent cultures are described; none have yet been successful.
Tipo: Text Palavras-chave: Dinophysis; Dense-cell suspension; Attempted culture.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00258/36956/35627.pdf
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Observations on possible life cycle stages of the dinoflagellates Dinophysis cf. acuminata, Dinophysis acuta and Dinophysis pavillardi ArchiMer
Berland, Brigitte R; Maestrini, Serge Y; Grzebyk, Daniel.
Some aspects of the life-cycle have been investigated in Dinophysis cf, acuminata, the dominant species of the genus along the French Atlantic coast, as well as in D, acuta; a few observations have also been made on the Mediterranean species D. pavillardi. Dinophysis cells occur in 2 clearly distinguished sizes. Small cells typically had a theca thinner than large cells, and cingular and sulcal lists were less developed. Both small and large cells were seen dividing, producing 1 to 4 round intracellular bodies. Some of these round bodies in turn contained many small flagellated cells which escaped through a pore and swam rapidly. Their behaviour after release, and how they might give rise to vegetative cells, has not been observed thus far; we do not...
Tipo: Text Palavras-chave: Dinophysis sp.; Life cycle; Cyst.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00258/36957/35626.pdf
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Recent aspects of nutrition in the dinoflagellate Dinophysis cf. acuminata ArchiMer
Berland, Brigitte R; Maestrini, Serge Y; Grzebyk, Daniel; Thomas, Pierre.
No species of Dinophysis has yet been cultured in the full sense. To determine the photosynthetic capability in D. cf. acuminata, the dominant morphotype of the genus along the French Atlantic coast, we took material from natural populations, in some trials by pipetting out single cells, and in others using plankton assemblages enriched in D. cf, acuminata by size fractionation and reverse sedimentation. Inorganic carbon uptake was measured at temperatures from 11 to 23 degrees C and at a light intensity of 400 mu mol photons m(-2) s(-1). Total uptake was proportional to the number of cells and increased linearly with incubation time, the uptake rate showing a distinct maximum at 18 degrees C, while at 23 degrees C being only half this maximum....
Tipo: Text Palavras-chave: Dinophysis cf. acuminata; Nutrition; Ultrastructure.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00258/36958/35624.pdf
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